Using Passive Flow Valves in a Micro-Fluidic System for LAMP Application

• Main Authors: Hung-Cheng Chang, 張宏誠
• Other Authors: 顏家鈺
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/99295283005434085671

博士 === 國立臺灣大學 === 機械工程學研究所 === 103 === This research presents the design of the passive flow valves in a microfluidic loop-mediated isothermal amplification (LAMP) system. It is possible to manipulate the microfluidic flow by carefully changing the balance among various participating forces including the Coriolis force, the centrifugal force and the capillary force. The Coriolis force is perpendicular to the tangential velocity, thus one can use slanted channels to change the flow direction. Changing disk spinning direction and velocity can change the balance between the capillary force and the centrifugal force. A multi-stage channel design was then implemented for a lab-on-a-disk biomedical diagnostic LAMP system. The lab-on-a-disk system consists of a servo system for centrifugal pumping and a heater for temperature control. To minimize the evaporative loss from the LAMP process, the system integrates the multistep reactor with heating capability to allow real-time LAMP execution. A polymer light emitting diode (PLED) section deposited on the disk then enables the turbidity test readout.