The Relation Between Storage Stability and Main Active Compounds from Polygonum cuspidatum High Resveratrol Content Selection 051111 Cultivated in Different Location

• Main Authors: Yuh-Wen Lai, 賴鈺文
• Other Authors: 張祖亮
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/03998241995236337694

碩士 === 國立臺灣大學 === 園藝暨景觀學系 === 103 === Polygonum cuspidatum Sieb. et Zucc. belongs to the family Polygonaceae, and it is used as a Chinese medicinal herb. The main active compounds including polydatin, resveratrol and emodin can be identified from the ethanol extract of its root and rhizome. Polygonum cuspidatum is known to have antioxidant activities and antitumor effects. These biological effects are related to the main active compounds. The tested plant of Polygonum cuspidatum ''051111'' line is selected by MingDao University for high levels of resveratrol. Biennial plants grown in Meifong highland region was harvest in June, July, August and October, respectively. The plants grown in greenhouse at National Taiwan University was harvest in January and April. Relevant researches of Polygonum cuspidatum are more emphasis on the individual efficacy of the main active compounds or overall effect of extract. However, there are lack of relations between overall effect and individual efficacy of compound. Therefore, this thesis is aimed at discussing the relation among content of compounds, antioxidant activities and cancer cell toxicity from different sources of Polygonum cuspidatum. In addition, the drying process and subsequent storage conditions of rhizome will if affect the content of main active compounds are investigated. The polydatin content ranges from 48.74±2.31 mg/g extract (Meifong June, which was planted in Meifong and harvest in June, same as here in the followings) to 71.28±0.41 mg/g extract (Taipei January). The resveratrol content ranges from 1.90±0.00 mg/g extract (Taipei January) to 14.60±0.53 mg/g extract (Meifong August). The emodin content ranges from 7.36±0.89 mg/g extract (Meifong July) to 12.67±0.81 mg/g extract (Meifong June). The content of total phenolics range from 198.16±16.26 mg GAE/g extract (Meifong October) to 269.02±8.93 mg GAE/g extract (Meifong August). The content of total flavonoid range from 13.84±2.4 mg QE/g extract (Meifong October) to 22.86±2.49 mg QE/g extract (Taipei January). Meifong August has the best DPPH and ABTS+ radicals scavenging activity. Meifong August and Taipei January have better FRAP reducing power. The total flavonoids contribute +56.72% to the DPPH radicals scavenging activity, and +40.33% to the FRAP reducing power. While the emodin content show 41.94% to the FRAP reducing power and -46.43% to ABTS+ radicals scavenging activity. Polygonum cuspidatum of Meifong August and Taipei January have cytotoxicity to HepG2 cell. For A549 cell only Meifong August in high concentration (300 μg / mL) is slightly toxic. Since Meifong August has higher main active compounds, it has better effect in every assay. Two drying methods are used to compare the drying effects on main active compounds after rhizome harvesting. The polydatin is higher by freeze drying method, but the resveratrol and emodin are higher by oven drying method. The resveratrol was about 4.8 times higher by oven drying method. To store the rhizome powder in 40℃ and 50℃ oven, the content of polydatin and resveratrol seem unchanged during the 50 days storage, but the content of emodin is gradually decreased.