Stability Analysis Of Broccoli And Black Rot Survival Studies By PCR Detection

• Main Authors: Chin-Chih Tsai, 蔡金池
• Other Authors: 張龍生
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/67634090832552718266

碩士 === 國立臺灣大學 === 園藝暨景觀學系 === 103 === Broccoli, an important domestic vegetable crop adapts to cool climate. Heat stress will affect its appearance, yield and quality. Due to the summer high temperature broccoli can not be produced in domestic. In recent years, many breeders bred domestic heat tolerant varieties, which have good stability in high-temperature environment. Those varieties maintain crop traits better at high temperature. The purpose of this study is to analyze the stability of heat-tolerant broccoli varieties with traits or not, through the collection of six heat-resistant varieties with an existing commercial varieties of broccoli, field trials in three different seasons. Including a tip bud microscopic observation experiments . Trials were RCB designed, plots area is divided into four blocks, each block 12 samples, the line spacing is 60cm * 60cm. Head traits were recorded, including head weight, head diameter, stem diameter, bracteal leaf, projecting leaf, head shape, flatness, Days from transplant to harvest.In Summer trials ''excellent'' stagnanted, no head production, late curd initiation, decrease of yield and quality occurred in other varieties. In the yield of summer, ''Tsinghua III'', ''AV515'', ''AV530'' are significantly more. And the effects of seasonal and variety have a certain interaction, choosing seasonal varieties gets high yield. The resistant for decrease in quality of ''AV515'', ''AV530'' is not as good as ''B35'', ''B45'' and ''Tsinghua III''. Gene effect in head shape and flatness is much more than climate effect and their interaction. To pursue good head shape and flatness must choose varieties with good genes. Xanthomonas campestris pv. Campestris cause black rot of cruciferous vegetables is a worldwide disease. Bacteria survival in seeds. Produce healthy seed is the key strategies to prevent and control black rot, also to maintain the quality of broccoli production. So the detection of this pathogen can ensure the maintenance of healthy seed . Trials testing different annual production of broccoli seeds, obtained electrophoresis graph by PCR, using the specificity primer. And use the dish to observe the appearance of colonies. The results showed that the annual production of 2011 contaminated seed and consistent with the observation of the colony appearance. And there is no specific relationship between black rot with the annual detection rate and soaking temperature, because PCR detection of nucleic acid fragments, whether the object is whether the survival of bacteria will be detected, but the temperature can affect the degree of adhesion of the bacteria surviving rate.