| Summary: | 碩士 === 國立臺灣大學 === 森林環境暨資源學研究所 === 103 === The purposes of this study are to extract the volatile organic compounds from Abies kawakamii (Hay.) Ito leaf by solid phase microextraction sampling, hydrodistillation and supercritical carbon dioxide fluid extraction, to identify the constituents of these extracts by GC-MS analysis; and to evaluate the bioactive activities of essential oil and supercritical carbon dioxide fluid extracts. Results showed that monoterpenoids were the main constituents of volatile organic compounds (VOCs) from A. kawakamii leaf, including α-pinene, camphene, limonene, β-phellandrene, and bornyl acetate. The yield of essential oil from Abies kawakamii leaf was 1.45%, and the main constituents of essential oil were α-pinene, camphene, and bornyl acetate. The highest yield of supercritical carbon dioxide fluid extract (250 Bar, 50℃) from A. kawakamii leaf was 1.56%; and its main constituents were α-eudesmol, bornyl acetate, maltol, and caryophyllene oxide. According to the results of bioactivity assay, the essential oil and supercritical carbon dioxide extract from A. kawakamii leaf exhibited the antimildew activity, shrimp lethality activity, and the positive effect on lifespan extension in Caenorhabditis elegans.
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