| Summary: | 碩士 === 國立臺灣大學 === 食品科技研究所 === 103 === Obesity is caused by excessive accumulation of body fat, which is closely related to complex metabolic diseased. Adipogenesis is a key process in determining the number and size of mature adipocytes in the development of obesity. Curcumin (Cur), has been reported to inhibit on adipocyte differentiation. But other curcuminoids present in turmeric, demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC) have not been investigated whether they exhibit anti-adipogenic activity. Here, we investigated the effects of curcuminoids on adipogenesis and the molecular mechanisms in adipocyte differentiation. Our Oil Red O staining result revealed that 25 μM curcuminoids effectively suppressed accumulation of lipid in adipocytes. And BDMC may exerts more potent anti-adipogenic function than DMC and Cur. This anti-adipogenic function of BDMC was largely inhibited the early stage of adipogenesis through prevention of mitotic clonal expansion (MCE). We found that BDMC suppressed MCE was accompanied by reducing the mitogen-activated protein kinase (MAPK) signaling pathways activation. In particular, BDMC blocked the cell cycle at G0/G1 phase by regulating the protein expression of cyclin A, cyclin B and p21. Also, BDMC reduced the protein expression level of PPARγ and C/EBPα. Furthermore, we examined the anti-obesity effects of BDMC on obesity animal model. C57BL/6 mice were fed high-fat diet (HFD) and BDMC at same time. The result showed that the HFD supplement with 0.5 % (w/w) BDMC had significantly lower body weight gain and less adipose tissue mass. H & E staining result showed that the mice were fed BDMC could inhibit hypertrophy in adipocyte. In conclusion, BDMC had anti-adipogenic effect in vitro and anti-obesity in vivo. Our results suggested that BDMC has potential to be an anti-obesity functional food.
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