Biotransformation of Ginsenosides in Saccharomyces cerevisiae
碩士 === 國立臺灣大學 === 食品科技研究所 === 103 === Ginsenosides are one of the primary bioactive substrates in ginseng. However, ginsenosides have low bioavailability. The purpose of this research is to investigate biotransformation of ginsenosides in yeast, Saccharomyces cerevisiae. We incubated wild-type yeas...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2015
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/56322055201094587238 |
| Summary: | 碩士 === 國立臺灣大學 === 食品科技研究所 === 103 === Ginsenosides are one of the primary bioactive substrates in ginseng. However, ginsenosides have low bioavailability. The purpose of this research is to investigate biotransformation of ginsenosides in yeast, Saccharomyces cerevisiae. We incubated wild-type yeast cells in YPD with 1000 μg/mL commercial ginseng essence product. Yeast cellswere sonicated to disrupt cell walls and ginsenosides were analyzed using HPLC/ESI tandem MS. The results indicated a significant decrease in ginsenosides Rb1 from 0.69 ± 0.018 μM to 0.49 ± 0.038μM（p＜0.001）, an increase in Rd from 0.19 ± 0.006 μM to 0.44 ± 0.029 μM（p＜0.001）, and an decrease in Rg3 from 1.01 ± 0.087 μM to 0.70 ± 0.036 μM （p＜0.001）in the YPD medium after 6 days fermentation. Intracellular ginsenoside Rg3 was also detected. We therefore propose that Saccharomyces cerevisiae may have the ability to biotransform and uptake ginsenosides, and has the capacity to function as a ginsenosides transporter genes screening platform.
|
|---|