| Summary: | 碩士 === 國立臺灣大學 === 化學研究所 === 103 === The theme of this thesis focuses on the application of lipid bilayer-modified graphene field-effect transistors (G-FETs) for the detections of chemical/biological activities of membrane proteins. Compared with one-dimensional (1D) nanowires to be used as a conducting channel in FET biosensors, the two-dimensional (2D) graphene sheets of G-FETs possess a larger and more stable interface with lipid bilayers, thus providing a convenient sensing platform with advantageous device designs in biological studies.
The first part of this thesis describes the device fabrication of G-FETs using high-quality, large-area graphene sheets synthesized from chemical vapor deposition (CVD) reaction. With a specially designed CVD reactor, the as-synthesized graphene sheets were produced within a confined reaction space, which significantly reduces the nucleation density and makes the formation of large-area, high quality graphene sheets possible.
The second part of this thesis displays the biosensing capabilities of a lipid bilayer-modified G-FET. A lipid bilayer was deposited on a G-FET via a vesicle fusion method. In the studies, we have applied this lipid bilayer-modified G-FET to detect phospholipase D and cholera toxin. With electrical measurements of the lipid bilayer-modified G-FET, transfer-curve shifts and electrical conductivity changes can be obtained after interacting proteins come to react with the modified lipid bilayer. Our experimental results have demonstrated that G-FETs can serve as a sensitive platform for biorecognition and biosensing investigations, in particular, suitable for the study of biological activities of membrane proteins.
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