| Summary: | 碩士 === 國立臺灣大學 === 化學研究所 === 103 === A surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using HgTe nanostructures as the matrix has been employed for the detection of four 15-base oligodeoxynucleotides (ODNs) and their complexes with mitoxantrone (MTX) that is a common drug for treatment of acute myeloid leukemia (AML) patients. The major peaks for the four tested ODNs are genes found in AML patients at m/z values of 4571, 4586, 4610, and 4635, while they are at m/z values of 5017, 5031, 5055, and 5079 for their corresponding complexes with MTX. The ODN with m/z value of 4610 is assigned for a normal gene of AML, while the other three are single-base mutant ODNs. This approach allows detection of the tested ODNs at the concentrations down to 2 nM, showing their potential for diagnosis of AML. The dissociation constants values of the four tested ODN-MTX complexes determined by the SALDI-MS approach are similar and all in the μM level, which agree with that determined by applying a conventional absorption approach. Relative to the conventional approach, the SALDI-MS approach has advantages of simplicity, rapidity, reproducibility, and use of smaller amounts of ODNs and MTX.
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