Fabrication of decellularized adipose tissue/alginate composite microspheres with pASCs encapsulation for tissue engineering

• Main Authors: Hsin-Yi Mao, 毛馨儀
• Other Authors: Jiashing Yu
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/42246331386096179872

碩士 === 國立臺灣大學 === 化學工程學研究所 === 103 === Cell-based therapies, new and current regenerative treatment, can use stem cells to create living and functional tissues to regenerate and repair tissue and organs in the body. In peripheral arterial disease, it has been demonstrated that stem cell treatment can improve perfusion and stimulate neovascularization. However, it usually suffers from some deficiencies, like immune rejection and limited proliferation of implanted cells. In this study, we focus on the application of the decellularize adipose tissue (DAT), which is a native extracellular matrix (ECM). We believe that the biomaterial extracted from the organism itself have great potential as a scaffold materials for stem cell culture as well as new tissue construct formation, especially the native ECM and stem cell are from the same origin (adipose tissue). We used DATsol (DAT solution) combined with alginate to culture Adipose stem cells (ASCs). In this work, we extracted DATsol to mix in high and low molecular weight alginate and investigated. The 2-D in virto study of DATsol/alginate could promote cell adhesion and differentiation; ASCs, immobilized in DATsol/alginate microspheres, showed metabolic activity which with an overall viability higher than 80%. We believe that the use of adult stem cell therapy in ischemia patients allows for the development of new mature and stable capillaries in patients affected by the severe condition.