The applications of microfluidics in the interaction of dimensionality toward cardiac myoblast and adipose-derived stem cells differentiation ability

• Main Authors: Chih-Wei Chao, 趙志偉
• Other Authors: Jiashing Yu
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/63902892329295565387

碩士 === 國立臺灣大學 === 化學工程學研究所 === 103 === Dimensionality is a physical cue that has been discussed less frequently among the various physical cues that influence cell behaviors. In this study, we took the advantage of microfluidics to fabricate three-dimensional (3D) gelatin-based scaffolds to mimic the natural circumstances of cell growth in an organism. We further compared the differences of cellular behaviors between 2D and 3D conditions. The models used in the presented study are H9c2 rat cardiac myoblast and human adipose-derived stem cell (hASC), both of which have differentiation ability. H9c2 is able to differentiate into skeletal or cardiac myocytes. Therefore, in this series of experiments, we aimed at investigating how dimensionality contributes to different types of muscle differentiation. We have found that H9c2 cells tended to fuse into multi-nucleated skeletal myotubes in 3D scaffold and differentiated into cardiac cells on 2D plane. However, the influence of dimensionality on cardiac differentiation was far less than that of chemical stimulation. hASC possesses multipotent differentiation ability toward osteogenic, adipogenic, myogenic and chondrogenic lineages. Specifically, we investigated osteogenic and adipogenic differentiation capability of hASC under different dimensional conditions. According to our results, the 3D environment supported adipogenesis and the 2D condition promoted osteogenesis. Our future work will focus on finding the factors which influence differentiation capability on the matrixes in different dimensionality. Furthermore, the myogenesis and chodrogenesis will also be investigated.