In Vitro immunomodulatory Function of Fucoidan from Cladosiphon okamuranus

• Main Authors: Hou, Wen-Ping, 侯文萍
• Other Authors: Kong, Zwe-Ling
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/10436235989962664017

碩士 === 國立臺灣海洋大學 === 食品科學系 === 103 === Fucoidan refers to a type of polysaccharide which contains substantial percentages of L-fucose and sulfate ester groups. It has many physiological activities such as anti-tumor, immunomodulatory, anti-inflammatory, anti-clotting, anti-thrombotic, anti-viral, anti-oxidation, and anti-lipemia. In this study, fucoidan isolated from Cladosiphon okamuranus investigated for its immunomodulatory potential by using in vitro assays. The glycosidase inhibitory assay showed that fucoidan has the ability to inhibit α-glucosidase and α-amylase. In vitro, fucoidan has the ability to scavenge free radicals. The immune-stimulating activity of fucoidan was elucidated by using RAW264.7, Jurkat and HB4C5 cells. The result showed that fucoidan enhanced proliferation of RAW 264.7 cells under the influence of significantly decreased the level of Nitro-Blue tetrazolium chloride (NBT), restrained the secretion of IL-6 and TNF-α, stimulated production of IL-10 and also enhanced phagocytosis activity of RAW264.7. Fucoidan also enhanced HB4C5 cell proliferation and significantly stimulated secretion of IgM and does not affect the IgG, as well as stimulated interferon-γ production of Jurkat cell. Based on these results, fucoidan has a potential to be used as a natural reagent for immune regulation.