| Summary: | 碩士 === 國立臺灣師範大學 === 人類發展與家庭學系 === 103 === According to the report from the Ministry of Health and Welfare, Taiwan, 2013, human colorectal cancer is the third-leading cause of cancer death in Taiwan. Due to the complexity and high recurrent rate of cancer, as well as side effects of cancer treatment, improvement in chemotherapeutic regimens is urgently needed. Curcumin, safe in human, regulates multiple pathways to inhibit tumor progression, such as apoptosis, autophagy, inflammatory factor NF-B and oncogene Aurora-A. This study was proposed to investigate the effect of curcumin on cell cycle, apoptosis, autophagy, and protein expression and activity of NF-B and Aurora-A using human colorectal cancer HT-29 cells. The results showed that curcumin increased the expression of pro-apoptotic protein caspase 3 and autophagy protein LC3-II, suggesting that curcumin induced apoptosis and autophagy. Significant decline of IB and phospho-IBin whole cell lysates and NF-B in the nuclei were observed. Flow cytometric analysis found that the proportion of acidic vesicles increased in curcumin-treated HT-29 cells. Administration of BAF reduced curcumin-induced increment of the proportion of acidic vesicles. Overall, curcumin changed cell cycle, triggered apoptosis and autophagy, inhibited the growth of HT-29 cells, and inhibited expression of NF-B and Aurora-A. The use of BAF reduced curcumin-induced autophagy.
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