| Summary: | 碩士 === 國立臺灣師範大學 === 化學系 === 103 === E.coli SOS-RFP biosensors which express red fluorescent protein as a signal protein under the control of recA, sulA, and dinH gene promoters in SOS response were constructed for detection of DNA damage and evaluation of DNA damaging agents. Two kinds of DNA damaging agents, which were mitomycin C(MMC), and nalidixic acid(NA), were tested using our E.coli SOS-RFP biosensors cells. We used three techniques to detect responses of biosensors , including microplate reader(detection of whole cells), microscope, and flow cytometry(detection of single cells). The SOS-RFP bacterial biosensors provide a specific and simple method for detecting DNA damaging agents.
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