Online monitoring of the concentration levels of gases during fermentation process by gas chromatography/whistle detection technique

碩士 === 國立臺灣師範大學 === 化學系 === 103 === This study was conducted to a general understanding of the use of a milli-whistle as a gas chromatography (GC) detector in gas analysis.The milli-whistle is connected to the outlet of a GC capillary, and when the eluted gases and the GC carrier gas pass through it...

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Bibliographic Details
Main Authors: Ye, Guan-Fu, 葉冠甫
Other Authors: Lin, Cheng-Hung
Format: Others
Language:zh-TW
Published: 2015
Online Access:http://ndltd.ncl.edu.tw/handle/04557319314845213328
Description
Summary:碩士 === 國立臺灣師範大學 === 化學系 === 103 === This study was conducted to a general understanding of the use of a milli-whistle as a gas chromatography (GC) detector in gas analysis.The milli-whistle is connected to the outlet of a GC capillary, and when the eluted gases and the GC carrier gas pass through it, a sound with a fundamental frequency is produced. The sound wave can be picked up by a microphone, and after a fast Fourier transform, the online data obtained for frequency-change vs. retention time constitute a new method for detecting gases with LabVIEW(Laboratory Virtual Engineer Workbench).In order to improve the efficiency of fermentation, in this study, yeast strain, Saccharomyces cerevisiae, using electrostatic spinning technology coated inside to the PLLA (poly-L-lactide; PLLA) microtubule arrays film. Yeast activated in film will not be affected. The film can be recycled and continue to use, reduce the cost of making ethanol. In this study, the fermentation broth and yeast that has been coated inside to fibers put into the container together to ferment. Using of program control self-assembled solenoid valve, the fermentation process produces ethanol and carbon dioxide vapors, etc., at 5-minute intervals, automatically injected into the capillary column to separate and record. Experimental results show that the use of our developed device, we can immediately detect and track fermentation. The experiment apparatus can detect at least 4 days with fully automated. According to the amount of change in frequency, it can instantly detect the concentration of ethanol that is in fermentation broth。