Identification of 3'-hydroxygenistein as a potent melanogenesis inhibitor from biotransformation of genistein by recombinant Pichia pastoris

• Main Authors: Wei-Ming Tseng, 曾偉銘
• Other Authors: Te-Sheng Chang
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/21710004961941502049

碩士 === 國立臺南大學 === 生物科技學系碩士班 === 103 === A product resulting from the biotransformation of genistein by a recombinant Pichia pastoris was isolated and identified as 3'-hydroxygenistein, on the basis of mass,1H-NMR, and 13C-NMR spectrophotometric analysis. The maximal product concentration and the conversion yield of the biotransformation in a 5 L fermenter were 3.5 mg/L and 14%, respectively. The inhibitory effects of 3'-hydroxygenistein on tyrosinase activity were investigated in vitro using mushroom tyrosinase. The results showed that 3'-hydroxygenistein potently inhibited tyrosinase activity with an IC50 value of 15.9 M. Furthermore, the inhibitory effects of 3'-hydroxygenistein on melanogenesis were also investigated in vitro in cultured B16 melanoma cells, and it was shown that 3'-hydroxygenistein dose-dependently inhibited melanogenesis in non-toxic concentrations. In summary, the 3'-hydroxygenistein that was produced from genistein by the recombinant yeast was confirmed as a potent tyrosinase inhibitor and inhibited melanogenesis in B16 cells.