| Summary: | 碩士 === 國立清華大學 === 分子醫學研究所 === 103 === Skin is the first line of barrier to protect the body from pathogens. Patients suffering from burns, diabetes wounds and chronic skin wounds are susceptible to infections. Tissue engineering- based cell therapies have been used to promote wound healing and demonstrated to be more effective than conventional wound dressing. In this study, we examined whether adipose-derived stem cell (ADSC) spheroids, a simple three-dimensional (3D) substitute, can accelerate wound healing. First, we found ADSC co-cultured with the human intestinal epithelial C2BBe1 cells formed tube-like structure. We then investigated whether ADSC could promote cell migration using the scratch wound healing assay on monolayers of C2BBe1 cells. The result showed that wound healing was significantly enhanced after treatment with conditioned medium from ADSC. In addition, we found that ADSC-spheroids formation by hanging drop had higher cells growth rate, and ADSC conditioned medium reduced IL-1β level during inflammation. This study was followed by using the cutaneous excisional nude mice model. We covered the 8 mm-diameter wound on mice with a dressing consisting of ADSC-spheroids embedded in a collagen gel. The ADSC- spheroids collagen dressing treated-group displayed a significant faster healing rate after one week than its untreated counterpart. Data from histology sections of the wounds suggest that the quicker healing rates by ADSC-spheroids embedded collagen dressing treatment may be due to the reduction of inflammation in the wound healing process. The establishment of the ADSC-spheroids collagen dressing is beneficial to patients with skin wounds. The dressing is also simple to prepare and is convenient to be used in patients. Therefore, ADSC-spheroids collagen dressing may provide a clinical alternative to promote wound healing.
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