| Summary: | 碩士 === 國立清華大學 === 材料科學工程學系 === 103 === The purpose of this study is to investigate the application of carbon fiber in neural implant. We used PC12 cells as a model, and compared the differences of proliferation and differentiation of the cells cultured in tissue cultured plates and on carbon fiber. The results revealed that although carbon fiber is a non-cytotoxic material which can be adopted as a substrate for normally proliferating PC12 cells to, however it cannot enhance the adhesion and proliferation of PC12 cells cultured on it.
Using the intrinsic property of high electrical conductivity of carbon fiber, we designed a device to provide the electrical stimulation to the PC12 cells cultured on carbon fiber and found that the electrical stimulation with potential over 200 mV is harmful to PC12 cells. The electrical stimulation with potential 100 mV makes no negative influence to the viability of PC12 cells, but can guide the neurites to outgrow along the carbon fibers.
Applying electrical stimulation to the NGF-induced differentiated PC12 cells cultured on carbon fiber, we found that electrical stimulation has very limited influence on the neurite formation, but it can enhance the elongation of neurites. Cells without electrical stimulation, the neurite length is 56 ± 4.8 μm μm, cells with 24 hours electrical stimulation, the neurite length is 108 ± 6.8 μm μm, about 1.92 times of the cells without electrical stimulation, and cells with 48 hours electrical stimulation, the neurite length is 132 ± 5.1 μm μm which is about 2.35 times of the cells without electrical stimulation. This results reveled that electrical stimulation has positive effect on neurtie growth with the addition of NGF.
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