| Summary: | 碩士 === 國立中山大學 === 機械與機電工程學系研究所 === 103 === The formation and application of fibers and films based on the near-field electrospinning (NFES) and electrospray (ESP) technology are investigated in this research. The poly [ (γ-methyl l-glutamate) , PMLG] was used as based material and then the Poly [ (ethylene oxide), PEO] and surfactant were mixed with PMLG to fabricate PMLG solution with 21.05 wt% concentration. Next, with the NFES and ESP processes, the high voltage electric field and numerical control (NC) controller (movement speed: 60 mm/sec, path spacing: 250, 500, 750 μm) were controlled to make the PMLG droplet to induce the repulsive force and form Taylor cone. Then, the ordered PMLG nanofibers (diameter: 4.15-6.25 μm) and thin films which deposited on the substrate were fabricated. The orientation of dipole in the PMLG solution could be enhanced with the applying of electric field due to the fact that NFES and ESP processed positive impact on piezoelectric properties. Furthermore, the experimental results showed that the maximum peak voltage was 0.056 V when the PMLG piezoelectric fibers were attached to the flexible electrical measuring device and measured by vibrating test. Finally, PMLG fibers and films which was non-toxic biological material and possessed excellent piezoelectric characteristics
were stably fabricated on indium tin oxide (ITO) -coated glass substrate in order to observe the proliferation status of cell. According to the result of research, the PMLG with negative charge could weaken proliferation ability of cells. Therefore, the decrease of the distance between electrospun fibers (spacing: 250-750 μm) made the proliferation of mouse fibroblast cells (NIH3T3) slightly decrease (cell coverage with different spacing during 4 days: 66.42-88.38 %). Moreover, the diffusion of the NIH3T3 was significantly reduced or even halted (cell coverage with different films density during 4 days: 3.07-42.03 %) when density of ESP films increased (films coverage: 41.05-89.55%) which meant that it possessed inhibitory effect.
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