The Studies on Crystal Stability of Virus-like Particle from Dragon Grouper Betanodavirus

• Main Authors: Yen-fu Lin, 林彥甫
• Other Authors: Chain-Shing Lin
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/q7wm6j

碩士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 103 === Dragon grouper nervous necrosis virus (DGNNV), a betanodavirus, is the cause of viral nervous necrosis in dragon grouper (Epinephelus lanceolatus). In larvae and juveniles, mortality is up amount 100%. The capsid protein of the DGNNV form virus-like particles (VLPs) that were expressed in Escherichia coli. So far, Crystals of VLPs diffracted X-rays to ~7.5 Å resolution (Luo and Lin, 2014). In this study, VLPs purified in phosphate buffer were cross-linked with DSS grown to crystals of higher resolution of diffracted X-rays. Among poly-His tagged VLPs and point-mutation, C338C6H can not form crystals while N25R29A mutant resulted in bigger size. VLPs for studies of DGNNV VLPs protein structure and function.