| Summary: | 碩士 === 國立中山大學 === 生物科學系研究所 === 103 === Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) was used for the analysis of squamous cell carcinoma (SCC) of skin tissues. Frozen sections (12μm thick) were analyzed by means of MALDI-IMS with a spatial resolution of 100 μm. MALDI-IMS data were found to predominantly feature complex lipids, including sphingomyelin (SM), phosphatidylcholine (PC), and triglyceride (TG). Molecular constituents were identified based on their exact mass and MS/MS fragment spectra. Histological features were identified by comparison of the samples with hematoxylin-eosin stains. The distribution of TG and PC indicated a clear boundary between normal and malignant tissues. In addition, PC (34:2), m/z 796.5, and PC (34:1), m/z 798.5 were found in well differentiated SCC, PC (32:0), m/z 772.5 was found in moderately differentiated SCC, and PC (32:0), m/z 756.5 was found in poorly differentiated SCC. These ions were present with higher relative abundance in SCC skin tumor tissues, and may be useful indicators in surgical procedures. Direct tissue analysis by MALDI-IMS is an important technology for assessing the localization of molecular species and revealing the underlying molecular signatures indicative of disease. It has the potential to help histology examinations by providing faster and more accurate diagnosis.
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