Evaluating toxic and health effects from the oganic compounds in the classroom dust by luciferase reportergene assays.

• Main Authors: Wu, Chi-Wei, 吳啟瑋
• Other Authors: Chao, How-Ran
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/72679843159812454489

碩士 === 國立屏東科技大學 === 環境工程與科學系所 === 103 === Several studies indicated that indoor dust is composed of environmental contaminants including heavy metals, volatile organic compounds, polycyclic aromatic hydrocarbons, pesticides, polybrominateddiphenyl ethers, gram-negative bacteria, allergen, phthalates, dioxin-like compounds, and other endocrine disrupting chemicals (EDCs). Many adverse health effects from EDCs had been reported such as neurotoxicity, developmental and reproductive toxicity, primarily in children. The classroom, especially in an elementary school, is one major environment for the young children, and their long-term stay results to their main non-dietary exposure to indoor dust comprising persistent organic pollutants. Therefore, establishing background levels of EDCs in elementary classrooms and evaluating the risks to a child’s health are necessary. In the present study, we used two bioassays: the Ad-DR bioassay and the T47D-kbLuc assay, to determine toxic equivalency (TEQ) and estradiol-equivalents (EEQ) in elementary classroom dusts including normal classrooms (NR) and computer classrooms (CR), and evaluated their health risks. Our results observed that concentration of TEQ in NR and CR dusts were 1.99~155 and 1.88~106 ng-ADL-BEQ /kg d.w. respectively. Estrogenic activity in CR ranged 0.25~ 32.6 ng -EEQ /kg d.w higher than in NR (0.0389~ 9.10 ng -EEQ/kg d.w.). The acceptable daily intake (ADI) in this study was significantly lower than ADI ingestion published by World Health Organization; however, the cancer risk hazard quotient (HQ) of dioxins in four samples was higher than the suggestion value of US EPA (10-6). In conclusion, Ad-DR bioassay and T47D-kbLuc assay are feasible tools in determining TEQ and EEQ concentration in dusts. However, the use of this bioassay in the evaluation of health risks posed by dioxin contamination may result to overestimation of values, thus, this study focuses on the revision of correction parameters needed to improve this bioassay method.