| Summary: | 碩士 === 國立屏東科技大學 === 動物疫苗科技研究所 === 103 === Coccidium is a parasitic protozoon, belonging to the genus Eimeria of the family Eimeria. The main infected hosts of coccidian are chicks, which aged in 3-6 weeks. The clinical symptoms in the infected chicks include diarrhoea, bloody faeces, and inefficient feed conversion rate. The most serious is death. Coccidiosis has a severe economic impact on worldwide. S, T, E and G are important coccidia genes. Four translated protein can induce good immune response, and therefore critical for the development of vaccines. In this study, We have constructed S, T, E and G genes into pET-28a expression vector. The preliminary results indicate that the antibody titer of immunized rSGET is significantly higher than other groups. In the weight increase ratio, immunized rSGET is also higher than without immunized group after challenge with oocyst. Regarding to these results, rSGET may be potential for developing an effective subunit vaccine against Coccidium. In addition, to enhance immunity response we add the recombinant IFN- γ ( r IFN-γ) from our lab with rSGET for secondary animal experiment. IFN-γ plays the important role in innate immunity and adaptive immunity, especially that it is one of the immune factors to induce antibody production from B cell for TH1 response. In our result, it has significantly difference in body weight comparison to the group of immune rSGET &; rIFN-γ then challenge with coccidiosis that compare with immune rSGET group and challenge group. In mortality rate and pathologic changes, the result shows that the group of immune rSGET &; rIFN-γ was lower than other groups. These results suggest that the vaccine of rSGET combine with rIFN-γcan enhance immune
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