Antioxidant and liver cell protective activities of solvent extracts from banana bracts and male flowers

• Main Authors: Tseng, Guo-Hsiang, 曾國香
• Other Authors: Wu, Ming-Chang
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/25614112258645780416

碩士 === 國立屏東科技大學 === 食品科學系所 === 103 === Banana Musa spp. (AAA), a member of perennial herb, is the fruit of banana trees. It is one of the most common fruit all over the world due to its high production. According to the literature that the banana has a rich dietary fiber, potassium, magnesium and many other substances beneficial to humans. The banana also increases capacities of immunity, anti-oxidation and anti-cancer, improves cardiovascular health, lowers blood pressure and even affects the treatment of depression. Banana farmers remove the banana flower after banana tree bears fruits in order to control the qualities and nutrition of the banana fruits. Banana flower can be cooked as dishes or made into many other food production; however, tons of banana flower is treated as agricultural waste and dumped in Taiwan every year. In previous studies, banana fruits have showed the capacities of anti-inflammatory and hepatoprotective, but studies related to banana flowers, especially banana buds and bracts, are less than fruits. Therefore, this study was going to analyze total phenol, total anthocyanins, total flavonoids and antioxidant capacity of three solvents extract of banana buds and bracts, which are treated as agricultural wastes while the banana fruits are harvested. Moreover, this study analyzed banana buds and bracts protective ability on injured FL83B liver cells. The results showed that the methanol extraction of banana buds has the maximum contents of total phenol and total flavonoids while the ethanol extraction of banana bracts has the maximum contents of total anthocyanins. Moreover, the methanol extraction of banana buds also has the better antioxidant capacity via DPPH radical scavenging, TEAC and other antioxidant capacity analysis. Toxicity test of mice normal liver cells (FL83B) found that the samples can contribute a better cell growth. And in protection test of the alcohol-induced liver injury, 400μg/mL concentration of methanol extraction of banana buds has significant protection to the mice normal liver cells and their survival rate is up to 92.74%. Besides, 800μg/mL concentration of water extraction of banana buds, methanol extraction of banana bracts and water extraction of banana bracts have obvious results, their rates are up to 89.39%, 91.17% and 85.75% respectively. In conclusion, the banana agricultural waste, such as banana buds and bracts, has considerable potential to be developed as a wonderful antioxidant or a health care food to prevent alcohol injury. Moreover, the new usage of the waste can increase the economic value of agricultural products and also reduce waste.