Selective extraction of chiral thiol drugs using gold nanoparticles followed by capillary electrophoresis

• Main Authors: Chun Meng Chung, 鍾均孟
• Other Authors: Ming Mu Hsieh
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/35047956460090187906

碩士 === 國立高雄師範大學 === 化學系 === 103 === Abstract D-Penicillamine(D-PA) and DL-Tiopronin(DL-Tio) are thiol drugs widely used for treating many diseases. In this paper, a simple method for the detecting of DL-Tio and D-PA is described. Tween 20-capped gold nanoparticles (Tween 20-AuNPs) were used as sCctive probes for chiral thiol drugs from an aqueous solution. Tween 20-AuNPs selectively extracted and enriched the thiol drugs from a complicated matrix, because the Tween 20 capped layer effectively inhibited nonspecific adsorption. A 10.0 mL chiral thiol drug solution was extracted using 200× AuNPs (200 µL), and the extracted aminothiols derivatized with fluorenylmethyloxycarbonyl at pH 10.0 were detected using capillary electrophoresis (CE) with UV detection. After extracting and centrifuging, dithiothreitol was used for detaching the chiral thiol drugs from the AuNPs surface. On-line concentration and separation of the detached chiral thiol drugs were performed using 25 mM β-cyclodextrin and 0.5%(v/v) polyethylene oxide as an additive in CE. Under optimal extraction conditions, the limits of detection at a signal-to-noise ratio of 3 for four chiral thiol drugs ranged 1.8–4.0 nM, and satisfactory linearity was obtained at 7.5–3000 nM (R2 > 0.996). These results suggest that the present method is inexpensive, rapid, easy, accurate.