| Summary: | 碩士 === 國立高雄師範大學 === 化學系 === 103 === Dispersive liquid-liquid microextraction followed by viscosity and field-amplifed sample stacking in capillary electrophoresis was determined to be a simple, fast and sensitive analytical method for simultaneously detecting three phenothiazines (promazine, chlorpromazine, and triflupromazine) in a human urine sample. The separation buffer was composed of 40mM phosphate solution (pH 2.5) and 10% (v/v) glycerol. Because glycerol exhibits viscosity, the resolution of the samples and the effect of the stacking could be substantially improved. Under optimized conditions, enrichment factors were obtained that ranged from 1190 to 2067. The proposed method for promazine, chlorpromazine and triflupromazine provided a good linearity that ranged from 0.75 to 200 nM, with correlation coefficients (r) ranging from 0.9975 to 0.9984. The limits of detection based on a signal-to-noise ratio of 3 ranged from 0.15 to 0.22 nM. The relative standard deviations of the target analytes were below 5%. The recoveries of three phenothiazines for urine samples at spiking levels of 50 to 200 nM were 87.0%~106.8%. With the advantages of a short analysis time, high enrichment factors, and high precision and accuracy, the developed method was successfully applied to determine phenothiazine drugs in human urine samples.
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