Development of thermal sensitive and disintegratable bio-film

• Main Authors: Kuang-Wen Chen, 陳廣文
• Other Authors: Hui-Huang Chen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/37767129604527635301

碩士 === 國立宜蘭大學 === 生物技術與動物科學系 === 103 === This study is to develop a thermal sensitive and disintegratable non-carrier bio-film which could be melted by skin temperature and could reduce waste disposal problems of the facial mask. Heat-unfolded fish skin gelatin (HG) and peptide of hydrolyzed heat-unfolded gelatin (HGP) were applied as the major material. Chitosan (Chi) and hydroxypropyl methylcellulose (HPMC) were applied as film-forming aids, glycerin (Gly) and sorbitol (Sor) were applied as plasticizer to improve mechanical and thermal melting properties of films. The molecular weight of HG was 66~16 kDa after 2 hr heating at 121oC. The structure transition temperature (Ts) decreased from 74.9 ℃ (gelatin) to 55.1 ℃ (HG2) in DSC thermograms. The HG was then hydrolyzed with combination of Alcalase and Pronase E. The fewer enzymes were utilized for HG than those for gelatin and more HGP with 11~4 kDa were obtained. The HGP was blended with HG with a ratio of HGP:HG=2:3 to facilitate the casting (desirable flow properties), film forming and stripping process. The combination of Chi/HPMC were applied as film-forming aids since they can lessen the Ts (63.4℃) and increase warm water solubility (86.8%) of films as compare with individual Chi or HPMC added films. The mechanical strength and the extensibility of films were improved by adding combination of Chi/HPMC and 10% Sor/Gly, respectively. The tensile strength and elongation at break of films were 1642 kPa and 111%, respectively. According to the image analysis, the area of thermal sensitive bio-film increased 40% within 10 min at 35℃, reflected the hydrophilic Chi/HPMC and Sor/Gly improved the thermal melting of film which could be melted around skin temperature.