Isolation, Identification and Preservative Tolerance of Wood Decay Fungi from Outdoor Wood Construction.

• Main Authors: Fang-Yu Lin, 林方瑜
• Other Authors: Sheng-Fong Lo
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/87614762189414633551

碩士 === 國立宜蘭大學 === 森林暨自然資源學系碩士班 === 103 === The wood decay fungi were identified with molecular methodology using the internal transcribed spacer (ITS) region and 28S ribosomal DNA. The nucleotide sequences were matched with the DNA sequence database of National Center for Biotechnology Information (NCBI), revealed Amyloporia sinuosa、Antrodia oleracea、An. wangii、Auriporia aurulenta、Ceriporia inflata、C. lacerata、Dacryobolus sudans、Fomitopsis ostreiformis、Gloeophyllum striatum、Hyphoderma praetermissum、Hyphodontia palmae、Marasmius haematocephalus、Peniophora lycii、Perenniporia tephropora、Phanerochaete sordida、Ph. stereoides、Pycnoporellus alboluteus、Schizophyllum commune and Skeletocutis diluta, total 17 genus 16 species. ACQ-1 and CA wood preservative were used to test inhibitory effects of mycelial growth selected test fungi before decay resistance test. The results show that four decay fungi, An. wangii、Sk. diluta、C. lacerate and F. ostreiformis, could accept higher concentration of ACQ-1 or CA preservative. According to CNS 6717 "Wood preservative performance benchmarks", the decay resistance test results show that An. wangii and F. ostreiformis meets the requirements specified in CNS 6717 when the retention requirements of ACQ-1 and CA preservatives up to K3 hazardous class standard. The An. wangii and F. ostreiformis exhibit high levels of tolerance for ACQ-1 and CA preservative in laboratory assays. Oxalic acid was a common metabolite of wood decay fungi that has been implicated in copper tolerance, the An. wangii and F. ostreiformis may have the similar physiological properties that can be used as a wood preservative application, detection and preservation in future.