Study on the Effect and Mechanism of Acteoside and SY-1 in Promotion of Wound Healing.

• Main Authors: Chen, Hung-Yueh, 陳宏岳
• Other Authors: Chang, Tsu-Chung
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/58958067475795018904

碩士 === 國防醫學院 === 生物化學研究所 === 103 === Background: Skin is the largest organ of human which functions to protect our body against external environment from infectious or toxic agents and excessive water loss. With the essential role of skin in protection of human body, it is critically important to maintain a healthy and intact skin.Previously, literatures documented that acteoside and the extract from Antrodia cinnamomea displays significant anti-oxidative and anti-inflammatory activites in skin cell models. Despite of the significant beneficiary effects, the effect and mechanism of these agents remain to be investigated. In an effort to explore the potential applications of these agents in skin protection, the effect and mechanism of acteoside and the extract from Antrodia cinnamomea in skin wound healing and hair growth were studied. Materials and Methods: The human dermal fibroblasts (HDF) and C57BL/6J mouse models were used for this study. The effects of acteoside and SY-1, a phenolic compound isolated from Antrodia cinnamomea, on anti-inflammatory, wound healing, and hair growth were investigated. The non-infectious and infectious models were performed to assessed the efficacious of these agents in wound healing. To investigated the mechanism of action of these compounds, western blot analysis were used to explore the expression of growth factors, inflammatory cytokines that are associated with skin inflammation and wound healing. Results: Our results indicated that acteoside and SY-1 displayed a significant anti-inflammatory activity in human HDF cells without detectable cytotoxicity. These compounds suppressed the level of TNFα-induced pro-inflammatory cytokines including IL6, IL1β, and NF-κB in HDF cells. In addition, the expression level of vascular endothelial growth factor (VEGF) and transforming growth factor β (TGFβ) were induced by these compounds. Furthermore, these compounds conferred a significant increase in wound healing and hair growth activity in both of the in vitro HDF cells and in vivo mouse models. Conclusion: This study demonstrated that acteoside and SY-1 display a significant skin protective effect through anti-inflammatory, increased wound healing, and hair growth promoting activities in human HDF cell and animal models. These findings provide the molecular basis for the skin protective activity and potential dermal applications of acteoside and SY-1.