| Summary: | 碩士 === 國防醫學院 === 生物化學研究所 === 103 === Ultraviolet B from the sun is widely considered as the major cause of human skin aging and enhanced collagen degradation. Metalloproteinases (MMPs) and reactive oxygen species (ROS) are increased by UVB treatment. Baicalein, isolated from the roots of Scutellaria baicalensis, is used for protection of photoaging. Baicalein is not only a ROS scavenger but also an inhibitor of 12-Lipoxygenase, has the ability of anti-inflammatory and anti-oxidant. 12-Lipoxygenase catalyzes arachidonic acid to 12-HETE which is an activator of TRPV1 channel.
In this study, we demonstrated that UVB increase MMP-1protein level in calcium-dependent manner but not ROS. We confirmed that 12-HETE increase intracellular calcium via TRPV1 ion channel. Treatment TRPV1 blocker can also cause decreased MMP-1 by UVB irradiated. Baicalein can reduce MMP-1 protein level through inhibit 12-HETE generation . On the other hand, silence TRPV1 channel can reverse affect by UVB irradiated.
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