Screening and identification of the antioxidants in Sarcandra glabra（Thunb.）Nakai by on-line HPLC-preanodized SPE/ESI-MS

• Main Authors: Yi-Jyun Hu, 胡宜君
• Other Authors: Kuo-Lung Ku
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/72420053389589951163

碩士 === 國立嘉義大學 === 應用化學系研究所 === 103 === Sarcandra glabra （Thunb.）Nakai has a history of thousands of years, currently not only widely used in the treatment of common diseases, its efficacy has even been used in the development of anti-cancer efficacy of clinical for gastric cancer, colorectal cancer, liver cancer, esophageal cancer and so on. The plant is the most important bioactive components, namely polyphenols. Such compounds classified into phenolic acids and flavonoids, both have the ability to scavenge free radicals and helps benefited anti-bacterial, anti-inflammatory, anti-aging effect. Based on the above described, because of the medical benefits of Sarcandra glabra （Thunb.）Nakai itself with the extremely high. Thus, the present is used on-line HPLC string of electrochemical detector with disposable screen printed electrode (on-line HPLC-preanodized SPE/ESI-MS), the effective separation of analytes to electrochemical synchronous detection antioxidant capacity of the individual peaks, combined with preanodization process screen printed carbon electrode, an electrode to improve detection sensitivity, while the material identified by mass spectrometry antioxidant activity of analysis. In this study, the solution composition, to explore the best preanodization of the conditions of reaction time and other parameters. From the experimental results that, at 10 ppm HNO3 aqueous environment, to impose the oxidation potential of 2 V, preanodization electrode preanodization processing time of 300 seconds under optimal conditions. Use the system successfully detected Sarcandra glabra（Thunb.）Nakai aqueous extract having quinic acid, caffeic acid, 3-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, caffeoylshikimic acid, 4-O-caffeoylquinic acid , rosmarinic acid-4-O-β-D-glucoside, rosmarinic acid, fraxidin, (2R/2S)-naringenin-6-C-β-D-glucopyranosyl-(6 1)-apiose, isofraxidin, quercetin-3-O-β-D-glucuronide, astillbon isomers and 8β,9α-dihydroxylindan-(5),7(1)ieb-8α,12-olide and other antioxidant substances. And after screen printed electrode pretreatment of preanodization, has improved significantly for the electrochemical signal of the effect. Since the detection sensitivity improved, making the original in the screen printed electrode to detect the analyte unmodified, preanodization of the electrode has been detected. The present study analyzes the success of improvement in the past when faced with a natural product ingredients, screen printing electrodes and increased detection sensitivity analysis in the electrochemical detection capabilities.