Study on the optimal pretreatment conditions of Chanos chanos scale collagen hydrolysate

• Main Author: 蔡幸珊
• Other Authors: 翁義銘
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/59113952626478965293

碩士 === 國立嘉義大學 === 食品科學系研究所 === 103 === The collagen enzyme hydrolysate one after another was already confirmed has the special effect, including the elimination of free radical, the oxidation resistance, the promotion of immunity, falls the blood pressure, the treatment of pain, the resist bacteria and so on. But humanity because worried the bovine spongiform encephalopathy as well as the transmissible spongiform encephalopathy threat, in recent years, all was partial from the aquatic product, for example jellyfish, squid, each kind of dried shark skin as well as reject internal organs, fish bones, scale, extract collogens or the enzyme hydrolysate and so on, in which received by the scale focuses attention on, not only safe but also might enhance the additional value and the utilization of waste, and might improve the questions of environmental pollution, it may be said killed several birds with one stone. Because the scale all has the black membrane as well as the fish silver generally adheres to stick cohere, contains melanin as well as fat when extract collagen enzyme hydrolysate front must consume the huge manpower, the physical resource, the funds carries on processing to be able to give to remove, therefore, this research main purpose is in the discussion milk-fish scale in the extract collagen enzyme hydrolysate best front process condition, simultaneously determines the extract collagen enzyme hydrolisis fluid oxidation resistance ability. Discovered from this research experimental result, (1) the best pretreatment condition, the elimination scale adheres to stick cohere the black membrane and the fish silver use the pattern which the heating and the lye and use to be best, in which heats up 75℃, pH=12 is best, the scale and the lye material fluid ratio is 1:4. (2) when (alkalinity protein decomposition enzyme) carries on scale hydrolysate processing by liquid state Alcalase, by 0.1% enzyme density most suits, in 50℃ under decomposes 8 hours effects to be best. (3) scale collogen hydrolysate effective extraction yield is approximately 50g/100g. (4) oxidation resistance result, the milk-fish scale enzyme hydrolysate (density 5%) is removing the DPPH free radical ability aspect with 0.05 mM Vitamin C, 0.05 mM BHA compares, is approximately 0.85: 0.93 : 1.0。(5) this research institute uses the milk-fish scale final hydroxyapatite survival rate is approximately: 35g/100g.