| Summary: | 碩士 === 國立嘉義大學 === 食品科學系研究所 === 103 === A high level of serum resistin has recently been found in patients with certain cancers, including colorectal cancer (CRC). Hence, it has been suggested that resistin may play a role in cancer pathogenesis and progression. Fulvic acid (FA), a class of compounds of humic substances, possesses various pharmacological properties; however, the effect of FA on cancer development remains unclear. In this study, we investigated the
effects of resistin on endothelium adhesion of CRC and determined whether FA elicits the antagonistic mechanism to inhibit these resistin effects. Human HCT-116 CRC cells and human umbilical vein endothelial cells (HUVECs) were used for all the experiments. We found that treatment of cells with resistin induced NF-κB activation and then ICAM-1 and VCAM-1 expressions in HCT-116 cells. Moreover, this induction finally promoted the adhesion of HCT-116 cells to HUVECs. However, co-treating the cells with FA and resistin demonstrated that FA significantly attenuated resistin-increased NF-κB activation, ICAM-1/VCAM-1 expression, and finally HCT-116 cell adhesion to HUVECs. These results clarify that resistin promotes HCT-116 cell adhesion to HUVECs; however, FA initiates the antagonistic effect to inhibit these resistin responses. Our findings, which provide new insights into understanding the promoted role of resistin and the antagonized effects of FA on the endothelium adhesion of CRC, suggest that FA may be a promising molecule for a therapeutic strategy against CRC metastasis, and should be taken into account in tumor therapy and management.
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