| Summary: | 碩士 === 國立嘉義大學 === 水生生物科學系研究所 === 103 === The objective of this study were : (1) to investigate the elimination half-lives of Oxolinic acid (OA) in bullfrog, Rana catesbeiana, muscle and liver in different methods of single administration, (2) to investigate OA distribution and elimination in tissues of bullfrog which were fed with OA at the dose of 30 and 60 mg/kg/day for five days.
A single dose pharmacokinetic study of oxolinic acid was applied by oral administration at the dose of 30 mg/kg. Oxolinic acid in bullfrog of peak concentration (Cmax) was obtained directly in the concentration time profile; the residue levels in muscle and liver samples after 12 h depletion were at the maximum concentration (muscle 86.9 μg/g, liver 122.0 μg/g), the second maximum concentration at 24 h (muscle 53.0 μg/g, liver 80.0 μg/g), then the OA concentration decreases with time. The half -life of muscle and liver were calculated to be 41.7 h and 29.32h. A single dose pharmacokinetic study of oxolinic acid was applied by intramuscular at the dose of 15 mg/kg. Oxolinic acid in bullfrog of peak concentration (Cmax) was obtained directly in the concentration time profile, the results showed that the concentration of OA in bullfrog muscle were 33.0, 14.9, 42.6 and 26.8 μg/g after injection at 1 h, 12 h, 24 h and 72 h, respectively. OA concentration of bullfrog muscle decreases with time after 72 h. The residue levels in liver samples after 4 h depletion were at the maximum concentration (68.0 μg/g), the second maximum concentration at 24 h (30.6 μg/g). The half-life of muscle and liver were calculated to be 25.7 h and 36.8 h.
The pharmacokinetics of oxolinic acid was applied by oral administration at the dose of 30 and 60 mg/kg/day, and was given at a daily dose for five consecutive days. The 30 mg/kg treatment at 14 days after single administration, the concentration of OA in muscle to 0.02 μg/g, and the concentration in liver to 0.04 μg/g, less than the statutory residual amount (0.1 ppm), at 28 days that can not be detected. The high-dose group at 28 days after dosing, the concentration of OA in muscle to 0.02 μg/g, and the concentration in liver to 0.04 μg/g, less than the statutory residual amount (0.1 ppm), at 35 days that can not be detected. According to these results, we suggested the withdrawal period, the one and half times days of OA in tissues below than MRL value, on muscle and liver of bullfrog were 21 or 42 days and 21 or 42 days, respectively.
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