Endometriotic Derived Mesenchymal Stem Cell Enhanced Sciatic Nerve Regeneration Using Absorbable Thread

• Main Authors: Che-Yu Liu, 劉哲宇
• Other Authors: Nancy M. Wang, Ph.D
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/35187423171282889892

碩士 === 國立彰化師範大學 === 生物技術研究所 === 103 === The peripheral nervous system injury would cause anomalous sensor or motor nerve further lifelong disability seriously. There were some treatment treatments for peripheral nerve injury, including direct suturing, nerve grafts and nerve conduits at present. Recently, studies have focused on conduits combined with cells; especially transplant Schwann cells to a sciatic nerve injury site for regeneration. Schwann cells had been proved can differentiate from mesenchymal stem cells in vitro, but had resource problem and immune rejection. The main purpose of this project is solving cell resource and nerve regeneration through endometriotic cell. Moreover, we would discuss cell differentiation mechanism. First, we would confirm the mesenchymal stem cell characteristics of endometriotic cell and Schwann cell specific marker Nerve growth factor receptor (NGFR) identify differentiated cell. To apply to animal model, we transect and remove the right sciatic nerve (10 mm) of Sprague-Dawley rat and implant 1×106 cells to injury site then fix with absorbable thread. We calculated weight, nerve diameter, nerve stimulates, and muscle atrophy to analysis. The results show that fold of withdraw reflex was 11 folds, muscle decrease 65% atrophy, regeneration nerve diameter was 1.7 folds compare to control and thread was absorbed by itself. In next generation sequencing (NGS), we suggest that endometriotic cells can prove axonal regeneration in injured nerve. On the other hand, Wharton's jelly cells control cell survival.