Transport of intensity equation from different wavelengths for quantitative phase imaging

碩士 === 國立中央大學 === 光電科學與工程學系 === 103 === Microscopy can be utilized to observe biological samples, but conventional wide-field microscopy cannot obtain phase information. Phase contrast microscopy has been invented by Zenike at 1953 to improve the contrast of samples. Although a phase contrast micros...

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Main Authors: Cheng Lin, 林晟
Other Authors: Cheng-Chung Lee
Format: Others
Language:zh-TW
Published: 2015
Online Access:http://ndltd.ncl.edu.tw/handle/48784713938480245770
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spelling ndltd-TW-103NCU056140452016-08-17T04:23:15Z http://ndltd.ncl.edu.tw/handle/48784713938480245770 Transport of intensity equation from different wavelengths for quantitative phase imaging 多波長光強度傳播方程之相位成像 Cheng Lin 林晟 碩士 國立中央大學 光電科學與工程學系 103 Microscopy can be utilized to observe biological samples, but conventional wide-field microscopy cannot obtain phase information. Phase contrast microscopy has been invented by Zenike at 1953 to improve the contrast of samples. Although a phase contrast microscope can significantly enhance the contrast of fine features, in general it cannot obtain the quantitative information from a biological objects. Transport of intensity equation (TIE) is a unique technique for phase imaging. Unlike phase contrast microscopy, it acquires quantitative phase information of samples. Here, we apply TIE with multiple wavelengths to reconstruct quantitative phase imaging. In our measurements TIE imaging with multiple wavelengths can retrieve quantitative phase information of fine features of sample in a fast fashion without scanning and has better quality than traditional TIE , also has real time possibility. Cheng-Chung Lee Yuan Luo 李正中 駱遠 2015 學位論文 ; thesis 90 zh-TW
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language zh-TW
format Others
sources NDLTD
description 碩士 === 國立中央大學 === 光電科學與工程學系 === 103 === Microscopy can be utilized to observe biological samples, but conventional wide-field microscopy cannot obtain phase information. Phase contrast microscopy has been invented by Zenike at 1953 to improve the contrast of samples. Although a phase contrast microscope can significantly enhance the contrast of fine features, in general it cannot obtain the quantitative information from a biological objects. Transport of intensity equation (TIE) is a unique technique for phase imaging. Unlike phase contrast microscopy, it acquires quantitative phase information of samples. Here, we apply TIE with multiple wavelengths to reconstruct quantitative phase imaging. In our measurements TIE imaging with multiple wavelengths can retrieve quantitative phase information of fine features of sample in a fast fashion without scanning and has better quality than traditional TIE , also has real time possibility.
author2 Cheng-Chung Lee
author_facet Cheng-Chung Lee
Cheng Lin
林晟
author Cheng Lin
林晟
spellingShingle Cheng Lin
林晟
Transport of intensity equation from different wavelengths for quantitative phase imaging
author_sort Cheng Lin
title Transport of intensity equation from different wavelengths for quantitative phase imaging
title_short Transport of intensity equation from different wavelengths for quantitative phase imaging
title_full Transport of intensity equation from different wavelengths for quantitative phase imaging
title_fullStr Transport of intensity equation from different wavelengths for quantitative phase imaging
title_full_unstemmed Transport of intensity equation from different wavelengths for quantitative phase imaging
title_sort transport of intensity equation from different wavelengths for quantitative phase imaging
publishDate 2015
url http://ndltd.ncl.edu.tw/handle/48784713938480245770
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