| Summary: | 碩士 === 國立中央大學 === 光電科學與工程學系 === 103 === Microscopy can be utilized to observe biological samples, but conventional wide-field microscopy cannot obtain phase information. Phase contrast microscopy has been invented by Zenike at 1953 to improve the contrast of samples. Although a phase contrast microscope can significantly enhance the contrast of fine features, in general it cannot obtain the quantitative information from a biological objects. Transport of intensity equation (TIE) is a unique technique for phase imaging. Unlike phase contrast microscopy, it acquires quantitative phase information of samples. Here, we apply TIE with multiple wavelengths to reconstruct quantitative phase imaging. In our measurements TIE imaging with multiple wavelengths can retrieve quantitative phase information of fine features of sample in a fast fashion without scanning and has better quality than traditional TIE , also has real time possibility.
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