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• Main Authors: Xi-Hoing Chen, 陳錫宏
• Other Authors: Fa-Kuen Shieh
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/5an8xn

碩士 === 國立中央大學 === 化學學系 === 103 === The first part of my research is based on our previous report, which demonstrated the synthesis of annular-functionalized mesoporous silica with controllable size (namely, XC-CAR-10, where X represents the number of carbons in the diamines used) with a fabulous adsorption ability to metal ion owing to macrocyclic effect. Herein, we applied the annular-functionalized mesoporous silica for the lanthanide ion adsorption and further utilized the lanthanide-adsorbed mesoporous silica for phosphorylated protein purification by use of Immobilized Metal Affinity Chromatography (IMAC). It is worth noting that adsorption of 3C-CAR-10 towards ~290 µg/mg of Er3+ was achieved, and the Er3+-3C-CAR-10 showed the remarkable purification capability and selectivity of phosphorylated peptide with about 90% elution recovery. The second part of my research is based on our previous report, which demonstrated a de novo synthesis of enzyme@ZIF-90 (Zeolitic imidazolate framework-90) and retained the enzyme activity. However, the catalytic rate of the embedded enzymes was still much slower than the native ones. One of the probable reasons of the loss in catalytic rate was likely attributed to the mass transfer owing to the particle size of carriers. Thus, the rapid syntheses of ZIF-8 with nano- and micro- size are modified and optimized in order to determine the connection between the catalytic rate of the embedded enzyme and mass transfer with size-dependent effect.