The characteristic study of ratiometric fluorescent probe 4-MB for detecting peroxynitrite

• Main Authors: Wu, Pei-Yu, 吳培郁
• Other Authors: Wang, Yun-Ming
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/kvx36t

碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 103 === Peroxynitrite (ONOO-) is an important signaling molecule in organism. It is produced from nitric oxide and superoxide radical by the diffusion-controlled reaction. Peroxinitrite is a strong oxidant and good neucleophile, it can react with different biomolecules. It has been related to a list of disease such as cardiovascular diseases, circulatory shock, diabetes, neurodegenerative disorders, and cancer. However, the effects of ONOO- in physiology and pathology remain controversial. There has been a strong demand for reliable and real-time analytical methods for ONOO- detection. The aim of this project is to design and synthesis a new fluorescent probe for detecting peroxynitrite. The design strategy of this ONOO- fluorescent probe, 4-MB, is based on the peroxynitrite anion might react with this probe by nucleophilic attack. The response time of 4-MB to react with ONOO- is about 4 minutes. 4-MB also has a good selectivity for ONOO- over other biologically relevant RNS and ROS. The detection limit of 4-MB for ONOO- is 29.8 nM. Moreover, 4-MB is successfully applied to detection of exogenous and endogenous ONOO- in living cells.