Graphene oxide-based nanocarrier combined with pH-sensitive tracer for simultaneous sensing and pH-responsive oligonucleotide delivery

• Main Authors: Hsieh, Chia-Jung, 謝佳容
• Other Authors: Hsu, Hsin-Yun
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/5cd8z7

碩士 === 國立交通大學 === 應用化學系碩博士班 === 103 === Graphene oxide (GO), 2-D structure of carbon nanomaterial, has been used in several bio-applications, such as biosensor, tissue engineering, bio-imaging, and drug delivery. Herein, we constructed a GO-based nanocarrier combined with the pH sensitive fluorescence tracer for both pH sensing and drug delivery. A series of GOs with different degree of oxidation were investigated to optimize the adsorption of model drug—poly-dT30. In our scheme, the pH-sensitive rhodamine dye was first applied for cellular pH monitoring. Under acidic environment, protonated rhodamine dye emits fluorescence at 588 nm (ex= 561 nm). The rhodamine dye-triggerd competition reaction occurred when the dT30-GO nanocarrier was introduced, leading to the release of oligonucleotide and quench the fluorescence of rhodamine dye by GO. We found more oxidized GO is better candidate for drug carrier in vitro. Current results have indicated its potential for targeted RNAi delivery in acidic tumor milieu.