Study of fluorescence enhancement in photonic crystals with different bandwidths of resonant modes

• Main Authors: Wu, Yu-An, 吳諭安
• Other Authors: Huang, Chen-Shen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/18736793486266917206

碩士 === 國立交通大學 === 機械工程系所 === 103 === This thesis is attempted to develop a photonic crystal enhanced fluorescence(PCEF) platform to improve the detection limit for microarray applications. To date, many PCEF platforms use narrow band resonant mode structures to achieve higher enhancement because the laser is used as illumination source. However, the possibility of developing a broad-band resonant mode structures for broad-band light source illumination such as LED can be beneficial for compact fluorescence detection system. So, this thesis will discuss the PCEF with different bandwidths of the resonant mode. The works presented in this thesis includes: 1. Optimization the PC geometries based on Rigorous Coupled Wave Analysis(RCWA), 2. Fabrication of a large-area PC by replica molding and sputtering technique, 3. Evaluation of PCEF using commercial laser fluorescence scanner, 4. Comparison the PCEF with various bandwidths of resonance modes using angle resolved fluorescence set up. The results from commercial laser scanner indicate that using PC slide can enhance the fluorescence by 33x compare to the most commonly used glass slides. From angle resolved fluorescence set up, we found the fluorescence enhancement is positive correlation to the bandwidth of the resonance mode, but needs more experiments to verify the result.