Effect of pore size of three-dimensional porous titanium dioxide for hepatocyte cultures

• Main Authors: Wang, Wen-You, 王文佑
• Other Authors: Lin, Chien-Cheng
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/28645556303614627053

碩士 === 國立交通大學 === 材料科學與工程學系所 === 103 === PU foam with different pore size was soaked in titanium dioxide (TiO2) slurry to adsorb TiO2 on the foam. And the foam was sintered to produce three-dimensional (3-D) porous TiO2 specimens. Then rat hepatocytes were cultured on TiO2 specimens. The influence of TiO2 specimen with different pore size on culturing hepatocytes has been studied. X-ray diffraction (XRD) and scanning electron microscopy (SEM) have been used to analyze crystal structure and morphology of sintered TiO2 specimen. XRD result showed no second phase remained in specimen after sintering at 1450°C for two hours. SEM was used to observe the surface of specimen and pore size and morphology of cross-section. The pore size of sintered specimen depended on the pore size of PU foam and most of the pores were cross-linked. On the other hand, the number of uncross-linked closed pores in the specimen with smaller pores was increased due to the effect of surface tension. In the part of culturing hepatocytes, MTT assay, ELISA and optical microscope (OM) were used to analyze the growth, function and morphology of hepatocytes. The data indicated the highest survival of hepatocytes in the culture dish without TiO2 specimen for three days. TiO2 specimen affected the cell growth in the pore size-dependent manner. However, the function of hepatocytes assessed by its albumin production was better in the culture with TiO2 specimen, specifically in the large size pores. OM indicated the different morphology of hepatocytes in the culture with TiO2 specimen. In summary, TiO2 specimen affected the cell growth of hepatocytes, while the function of hepatocytes were relatively excellent as compared with traditional 2-D culture dish.