The characteristic study of hydrogen sulfide fluorescent probe MaT-cyclen

• Main Authors: Lee, Lu-Ying, 李律瑩
• Other Authors: Wang, Yun-Ming
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/cwy39w

碩士 === 國立交通大學 === 生物科技系所 === 103 === To design a selective and quick response method for monitoring hydrogen sulfide in aqueous media that works under the biological system. However, the applications of bioimaging studies or real sample detection of fluorescent chemosensors for hydrogen sulfide have been rarely reported, and even the reported methods have some disadvantages such as poor solubility in aqueous media and lack of reversible nature. The design of fluorescent sensors for hydrogen sulfide in aqueous solution is a challenging task owing to the strong hydration of anions, which weakens the interactions of the sensors with the target anions. But the displacement method can be used to tackle this hurdle. Based on the above mentioned facts, cyclen based chemosensor ligand, 1-((1-((10-methylanthracen-9-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)-1,4,7,10-tetraazacyclododecane (MaT-cyclen) was designed, synthesized and characterized. In this study, MaT-cyclen exhibited high sensitivity and selectivity towards Cu2+ in aqueous medium. MaT-cyclen also exhibited excellent optical properties and formed complex [Cu(MaT-cyclen)2] when combined with CuCl2, which displayed fluorescence ON–OFF behavior. This complex also showed high sensitivity and selectivity for HS- to show fluorescence OFF- ON behavior on the basis of forming CuS. In addition, [Cu(MaT-cyclen)2] displayed rapid response (<5 sec), well reversibility and high sensitivity for recognizing HS− in 100% aqueous medium. Furthermore, its potential utility for biological applications was confirmed by fluorescence imaging of hydrogen sulfide in living cells.