Cardiac Biomarkers Response to a Set of High-intensity Intermittent Exercise Bouts

• Main Authors: Lu, Kang-Hao, 陸康豪
• Other Authors: Chan, Kuei-Hui
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/85817316653526992099

博士 === 國立體育大學 === 競技與教練科學研究所 === 103 === Vigorous endurance exercise could result in the release of cardiac biomarkers into blood, including creatine kinase-MB isoenzyme (CK-MB), cardiac troponin (cTn), and N-terminal proB-type natriuretic peptide (NT-proBNP). Despite regular high-intensity intermittent exercise can improve both aerobic capacity and performance, whether this type of exercise may induce myocardial injury or overload is still unclearly. Purposes: 1. To determine the effect of acute high-intensity intermittent running on cardiac biomarkers in basketball players. 2. To compare the responses of cardiac biomarkers between high-intensity intermittent and moderate-intensity continuous exercise trials in healthy adults. Methods: Part 1-Ten male collegiate basketball players were recruited and completed seven 2-min intermittent running bouts at 90% VO2max separated with 1-min rest periods. Blood samples were collected at pre-, 0 h, and 24 h post-exercise for measuring CK-MB, CK, cTnI, NT-proBNP, ischemia-modified albumin (IMA) levels and the indicator of lipid peroxidation-thiobarbituric acid reactive substances (TBARS). Data were analyzed by repeated-measures one-way ANOVA or the Friedman test. Part 2-Sixteen healthy, adult males performed both intermittent (Trial I) and continuous exercise trial (Trial C) on an ergometric bicycle in a counter-balanced and crossover design. The total workout of Trial I was similar to Trial C. Trial I included 6 bouts of cycling at an average intensity of 85% VO2max with each bout lasting for 4 min and separated by 3-min rest periods. Trial C included 36 min of continuous cycling at ~57% VO2max. Blood samples were drawn at pre-, 30 min, 3 h, and 24 h post-exercise, and the biomarkers were including CK-MB, CK, cTnI, IMA, NT-proBNP, TBARS, and uric acid. A two-way ANOVA with repeated-measures was used to compare the differences of these biomarkers between trials and time points except for cTnI; the nonparametric statistics were used for cTnI data. Results: In Part 1, although the significant elevations in CK-MB and CK were found in basketball players following intermittent running (p < .05), there were no significant changes in levels of CK-MB/CK, cTnI, NT-proBNP, IMA, and TBARS (p > .05). In Part 2, there was a minor elevation of cTnI for some participants following Trial I but it did not reach the statistics significant level (p > .05). Uric acid concentrations were significantly increased following Trial I, and the data in Trial I was significantly higher than Trial C (p < .05). Plasma NT-proBNP levels were significantly increased after both types of exercise (p < .05) but the magnitude of effect was small; there were no significant changes in CK-MB, CK, IMA, and TBARS levels. Conclusion: The both types of running or cycling high-intensity interval exercise protocol for basketball players or healthy males designed by this study did not result in serious myocardial damage or overloading. The interval cycling might induce a transcint increase in oxidative stress. The release of CK-MB following intermittent running could be from skeletal muscle but not cardiomyocytes.