Studying the functional role of CPAP in IL-6/STAT3 pathway in HCC

• Main Authors: Chun-GuoGuo, 郭春國
• Other Authors: Liang-Yi Hung
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/60250230673318293737

碩士 === 國立成功大學 === 生物資訊與訊息傳遞研究所 === 103 === Overexpression of GFP-CPAP significantly enhanced STAT3-driven transcriptional activation in response to IL-6 treatment. Overexpressed GFP-CPAP transcriptionally increased the targeted gene expression of STAT3 upon IL-6 stimulation. Co-immunoprecipitation showed that CPAP can form a complex with STAT3 in response to IL-6 treatment. Immunoblot analysis further indicates that overexpression of CPAP is positively correlated with activated STAT3 (p-STAT3) in HCC tissues. On the other hand, it was reported that interleukin-8 (IL-8) can regulate angiogenesis by enhancing endothelial cell survival, proliferation and matrix metalloproteinases production. Interestingly, overexpression of GFP-CPAP enhances the promoter activity of IL-8 under IL-6 treatment. The enhanced expression of IL-8 and VEGF by overexpressed GFP-CPAP were further confirmed by ELISA. By orthotopic and splenic mouse model, it showed that overexpressed CPAP can promote tumor growth and metastasis. Moreover, the expression of CPAP is positively correlated with plasma IL-8 in HCC patients. Taken together, our results suggest that CPAP may contribute to HCC malignancy through the IL-6/STAT3 pathway-mediated IL-8 and VEGF expressions.