| Summary: | 碩士 === 國立成功大學 === 化學工程學系 === 103 === A label-free electrochemical hemoglobin biosensor able to rapidly respond to a wide-range of analyte concentrations (0.0001 to 1 mg/ml in PBS) is presented. Hemoglobin adsorption on a carbon paper electrode is detected by exploiting the high sensitivity and low background current of differential pulse voltammetry.
The results indicate that oxygen reduction on the carbon electrodes is facilitated leading to a positive potential shift by the oxygen-binding property of hemoglobin. Although the reduction peaks caused by adsorption of hemoglobin and myoglobin are harder to separate, interference from myoglobin was able to be minimized by measuring in an acidic environment. And further modification of electrode surface by surfactants immersion can dramatically decrease the myoglobin signal, thus Hemoglobin /Myoglobin signal ratio increased from 0.79 to 80. The response of the electrodes when challenged with bovine serum albumin (BSA), lysozyme is also reported, with no current response to BSA or lysozyme being detected. The SEM images indicate the presence of micro structures comprising shallow notches on the carbon electrodes that lead to an unequal distribution of absorbed proteins.
Since there are no electrical active probes or fluorescence-labeling chemicals involved, this platform offers a potentially safer route to in vivo hemoglobin measurement.
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