Development of three-dimensional DNA nanostructure for anti-bacteria strategy

• Main Authors: Ching-YiHsieh, 謝靜宜
• Other Authors: Dar-Bin Shieh
• Format: Others
• Language: en_US
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/fb9224

碩士 === 國立成功大學 === 口腔醫學研究所 === 103 === The rapid development of nanomedicine based on theranostic has gradually evolved into practical clinical applications. Recently, researchers have reported that certain inorganic nanomaterial may induce cytotoxicity and biological dysfunction. DNA is an intrinsic biological macromolecule thus their nanostructures were regarded as excellent carriers with low toxicity and high biocompatibility. In my study, I would like to develop an advanced theranostic platform based on a self-assembled DNA nanostructure as scaffold with targeting aptamer and carrying antibiotics. Antimicrobial molecules (Actinomycin D, AMD) will be attached to minor groove of DNA double helix through hydrogen bond. The DNA nanostructure system serves as carriers for targeting delivery of AMD to attack bacteria. Currently, the DNA nanostructure system has been assembled and was confirmed by DNA agarose gel electrophoresis. Cryo-electron microscopy (cryo-EM) and three-dimensional (3D) reconstruction technique showed the DNA nanostructure system was a hollow spherical structure. In addition, we found a single DNA nanostructure could carry 2409.47 AMD molecules when saturated. We further found that the anti-bacteria ability of our system had a higher efficacy than free AMD. In the aptamer binding assays, we demonstrated the aptamer target specifically to Streptococcus mutans.