TiO2-nanotube Surface for Investigation of Biocompatibility and Hemocompatibility

• Main Authors: Pei-Chieh Wong, 翁培傑
• Other Authors: Shu-Ping Lin
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/51290338618555160380

碩士 === 國立中興大學 === 生醫工程研究所 === 103 === Nanoscale metallic substrates for improved biocompatibility and hemocompatibility are a persistent theme in biomaterials research and biomedical applications. For example, the need to improve the long¬term prognosis of commercially available bare-metal stents has drawn a lot of attention. In this study, we focused on surface modification of titanium (Ti) for simultaneously investigating the effects of nanoscale surface structure and angstrom-scale chemicals on biocompatibility and hemocompatibility. The technique of electrochemical anodization was used to create uniform nanotubular structure of titanium oxide on the surface of titanium (ATN). After the process of anodization, the ATN samples were individually either coated by NO or modified by 3-mercaptopropyltrimethoxysilane (MPTMS). Ti, ATN, NO coated ATN (NO-ATN), MPTMS modified ATN (MPTMS-ATN), NO coated MPTMS-modified ATN (NO-MPTMS-ATN), and MPTMS modified NO-coated ATN (MPTMS-NO-ATN), were separately examined their material properties using electron spectroscopy for chemical analysis (ESCA) and contact angle goniometer. The results of ESCA showed the specific chemical bondings after each step of modification. In addition, all the surface modified samples displayed better hydrophilic property in comparison with Ti. Endothelial cells on NO-MPTMS-ATN and MPTMS-NO-ATN were respectively increased to 1190±14.74 cells/mm2 and 1167±24 cells/mm2 in five days of culture. Although the tests of prothrombin time (PT) and activated partial thromboplastin time (aPTT) exhibited normal in all samples, the fibrinogen concentration was 193.18±6.0 mg/dl on MPTMS-NO-ATN and less than other five groups and reference value. Collectively, the modified surfaces showed augmented proliferation of endothelial cells and improved hemocompatibility. This cost-effective and industrially-scalable technique of metallic surface modification can be successfully utilized to offer a cell/hemo-friendly solution to the inherent limitations pertinent to bare metallic stents in the future.