Rapid Detection of Ketamine in Oral Fluid Using Microfluidic Paper Based Analytical Devices

• Main Authors: Yu-Chun Yen, 閻侑君
• Other Authors: Chien-Fu Chen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/41518009916702411371

碩士 === 國立中興大學 === 生醫工程研究所 === 103 === A novel oral fluid testing platform based on microfluidic paper‐based analytical devices (µPADs) is proposed for on-site high-throughput monitoring of driving under the influence of drug (DUID). A key advantage of the proposed technology is the ability to simultaneously and effectively measure low concentration of ketamine in oral fluid without the need of complex equipment, power sources and long processing time. The detection system will provide key elements including (a) adequate, consistent and high recovery oral fluid collection without dilution, (b) selection of antibodies for high-specific immunoassay screening of specific drug classes, (c) high sensitive and high throughput µPAD fabrication and characterization for capturing and concentrating abused drugs in oral fluid and (d) surface modification for high density of different types of antibodies binding on cellulose substrates, offering a robust and rapid solution for on-site drug screening. As a result, a ketamine assay with a detection limit of 10 ng/mL, and a single assay can be completed in 6 min.