| Summary: | 碩士 === 國立中興大學 === 農藝學系所 === 103 === Hypericum perforatum L. and Hypericum coris L. have been taken as experimental materials in this study. Effects of exogenous melatonin on active compounds of H. perforatum and H. coris under different photoperiods and temperature / photoperiodic stresses have been investigated. Amounts of active ingredients of both experimental materials have been analysed by high performance liquid chromatography (HPLC) under 5 different photoperiods (L: D = 6 h: 18 h、12 h: 12 h、18 h: 6 h、10 h: 10 h and 14 h: 14 h). The contents of melatonin, hypericin, and quercetin of H. perforatum will increase with the lengthened periods of light phase. Whereas, the levels of melatonin and quercetin of H. coris will be elevated with the lengthened periods of light phase. In addition, the highest amounts of rutin of H. perforatum and H. coris were detected under the entrained (with Zeitgeber) condition of 20 h- photoperiod (10 h:10 h) with 11.10 μg / mg FW and 9.83 μg / mg FW, respectively.
In order to explore the effects of exogenous melatonin on resistance of H. perforatum and H. coris to stresses, the plants were cultivated under low temperature (10 ℃) and photoperiodic (L:D = 10 h:10 h) stress with application of 5 melatonin treatments (0 μΜ, 125 μΜ, 250 μΜ, 500 μΜ and 750 μΜ). It reveals that exogenous melatonin treatments can improve root growth of both experimental materials. At certain dosages (250 μΜ and 750 μΜ) of applied melatonin, the contents of endogenous melatonin and hyperforin of H. perforatum and H. coris can be elevated circa 1~2 folds.
For the production evaluation of H. perforatum under controlled environments of culture facilities, it exhibits that different sources and proportion of growth media would influence the accumulation of active compounds in this medicinal herb. Among different culture pots, styrofoam pot used for cultivation of H. perforatum has shown to be better. Otherwise, it shows that application of 10% Hoagland’s solution can improve the biomass and contents of melatonin, hyperforin and rutin of H. perforatum, but with no significant effects between frequencies of applied nutrient solution. Comparison of three different culture facilities for small-scale production evaluation of H. perforatum, it reveals that green house composed of natural lights assisted with artificial light sources is superior.
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