Ratio Computation of Stroke Rat Brain Infarct Volume and Atrophy Volume Relative to the Normal Brain Volume

• Main Authors: Ya-Lan Chang, 張雅嵐
• Other Authors: Yung-Kuan Chan
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/34004953410238103827

碩士 === 國立中興大學 === 資訊管理學系所 === 103 === According to recent research, stroke is one of the main causes of death as well as a source of massive economic burden that has concerned many researchers to work in the field of stroke research. Stroke is a cerebrovascular disease mainly divided into two types: ischemic stroke and hemorrhagic stroke, which refers to the partial blockage or bleeding inside blood vessels of the brain respectively. Both types of stroke lead to nutrient and oxygen deprivation in the brain, which ultimately cause brain damage or death. This study focused on ischemic stroke by using rats with middle cerebral artery occlusion (MCAO) as experimental subjects, and examining the images of their brains obtained from staining with 2, 3, 5-triphenyl tetrazolium chloride (TTC). The stroke rat brain infarct volume and atrophy volume computation system (SRBIAVC system) segmented the images via methods of background removal, gamma correction, brain center line detection, and genetic algorithm to yield a correct estimate on the volume of brain infarct and atrophy. This way, the SRBIAVC system can provide useful information in the development of new drugs for stroke treatment. In this study, the volume of infarct and atrophy was calculated based on 168 images of brain slices segmented from 28 original images of rat brains. As indicated by the experimental results, the estimates obtained by the SRBIAVC system were better than those by other methods and more closely approximated the estimates by experts.